Programme

Registration desk opens

Lunch

Meeting opening

Session 1: Endosomal recycling events

Chairs: Cesare Montecucco and François Darchen

Bertrand Cinquin (Paris, France)

Deciphering the recycling events by automatic analysis of TIRFm sequences (20')

Mireille Cormont (Nice, France)

Rab4b in the control of endosomal trafficking (20')

Damien Jullié (Bordeaux, France)

Recycling endosomes undergo kiss-and-run exocytosis in hippocampal neurones (20')

Julie Mazzolini (Paris, France)

Organisation of F-actin and membrane remodeling during phagocytosis visualised by TIRFM (20')

Coffee break

Session 2: Endosomal sorting and dynamics

Chairs: Alessandra Moscatelli and Christophe Lamaze

Guillaume van Niel (Paris, France)

Regulation of distinct endosomal sorting processes by the Tetraspanin CD63 (20')

Olga M. de Brito (London, UK)

A novel mechanism regulating Rab7 function in the endocytic pathway (20')

Andreas Mayer (Epalinges, Switzerland)

Vacuole fusion: SNAREs dissociate the V-ATPase to liberate V0 proteolipids and promote lipid mixing (20')

Richard Ruez (Paris, France)

Mechanical stress, caveolae and Jak/Stat signaling (20')

Coffee break

Session 3: Secretion pathways

Chairs: Franck Perez and Ricardo Borges

Gaelle Boncompain (Paris, France)

Cargo partitioning imaged using a synchronized secretory assay: the RUSH system (20')

Margherita Cortini (Milan, Italy)

ERp44 acts as a pH-dependent chaperone and retrieves client proteins from the Golgi complex (20')

Alexandre A. Mironov (Milan, Italy)

The way to a new paradigm of intracellular transport (20')

Stéphanie Lebreton (Paris, France)
Simona Paladino (Naples, Italy)

Role of oligomerization in apical sorting and plasma membrane organization of GPI-anchored proteins in polarized epithelial cells
(10’ each of last two speakers + 10’ shared question time; total time of the double talk 30’)

Dinner

Poster Session (Poster listings can be found here)

Session 4: Molecular machineries I

Chairs: Alessandra David Perrais and Vincenzo Sorrentino

Kristine Schauer (Paris, France)

Probabilistic density maps to study global endomembrane organization (20')

Klemens Wild (Heidelberg, Germany)

Molecular mechanisms of co- and post-translational targeting to the endoplasmic reticulum (20')

Nicola De Franceschi (Turku, Finland)

Longin domain and small GTPases: building blocks and ancient partners in the trafficking machinery (20')

Doris Ricotta (Brescia, Italy)

Functional phosphorylation events involving the epsilon/hinge ear domain of the ap-4 epsilon adaptin subunit (20')

Nathalie Pujol (Marseille, France)

An unusual STAT-SLC6 protein complex associated with endocytosis in epidermal innate immunity in C. elegans (20')

Presentation by Thermo Fisher

Coffee break

Session 5: Molecular machineries II

Chairs: Francesco Filippini and Klemens Wild

Frédéric Boal (Bristol, UK)

Function of Rip11 C2 domain in GLUT4-containing vesicles fusion in adipocytes (20')

David Perrais (Bordeaux, France)

A high precision survey of the molecular dynamics of mammalian clathrin-mediated endocytosis (20')

Vincenzo Sorrentino (Siena, Italy)

Molecular basis of protein localization to the junctional region of the sarcoplasmic reticulum in skeletal muscle cells (20')

Gian Pietro Di Sansebastiano (Lecce, Italy)

Time for a new iSNARE model launch (20')

Lunch

Session 6: Ubiquitinylation in trafficking

Chairs: Guillaume van Niel and Romeo Betto

Evelina Gatti (Marseille, France)

Ubiquitination and MARCH E3 ubiquitin ligases regulate the intracellular traffic of antigen presenting MHC class molecules (20')

Olivier Walker (Villeurbanne, France)

Tackling the interaction of stam2 with ubiquitin and polyubiquitin chains by structural and dynamical NMR studies. Cooperativity or not, that is the question! (20')

Sara Sigismund (Milan, Italy)

An analogical to digital ubiquitin-based switch controls EGFR fate (20')

Coffee break

Free time

Poster Session / Annual meeting of Club Exocytose-Endocytose
(Poster listings can be found here)

Dinner

Poster Session (Poster listings can be found here)

Session 7: Trafficking in neurons

Chairs: Claudia Verderio and Thierry Galli

Françoise Coussen (Bordeaux, France)

Profilin-IIa, an actin binding protein, regulates the trafficking of kainate receptors through its specific interaction with the GluK2b subunit (20')

Assunta Senatore (Rome, Italy)

Mutant Prion Protein Suppresses Glutamatergic Neurotrasmission in Cerebellar Granule Neurons by Impairing Membrane Delivery of Voltage-gated Calcium Channels (20')

Stéphane Ory (Strasbourg, France)

Compensatory endocytosis in chromaffin cells requires Scramblase1 activity (20')

Damiano Zanini (Padua, Italy)

A rosette of SNARE complexes in neuroexocytosis (20')

Rémy Sadoul (Grenoble, France)

Release of exosomes from differentiated neurons and its regulation by synaptic activity (20')

Coffee break

Session 8: Trafficking in immune cells and autophagy

Chairs: Ana Maria Lennon and Evelina Gatti

Federica Benvenuti (Trieste, Italy)

Regulation of cytokine secretion in dendritic cells at the immunological synapse (20')

Angélique Bobrie (Paris, France)

Role of RAB27 proteins in exosome secretion: implication in anti-tumor immune responses (20')

Paola Larghi (Paris, France)

Role of TI-VAMP in CD4 T cell functions (20')

Till Wenger (Marseille, France)

Regulation of autophagy and endogenous MHC II presentation by TLR signaling in dendritic cells (20')

Lunch

Session 9: Trafficking in plant cells

Chairs: Gian Pietro Di Sansebastiano and Patrick Moreau

Thibaud Adam (Dijon, France)

Constitutive expression of the C-terminal end of clathrin heavy chain inhibits cryptogein induced clathrin-mediated endocytosis in tobacco cells (20')

Alessandra Moscatelli (Milan, Italy)

Different endocytic pathways require the coordinated action of actin filaments and microtubules in tobacco pollen tube (20')

Béatrice Satiat-Jeunemaitre (Gif sur Yvette, France)

Effects of sphingolipid biosynthetic pathways inhibition on polarity, membrane trafficking and endomembranes organization in plant cells (20')

Session 10: Trafficking and disease

Chairs: Andreas Mayer and Simona Paladino

Diana Pendin (Padua, Italy)

Functional analysis of atlastin interacting partner Reticulon in Drosophila melanogaster (20')

Jocelyn Laporte (Illkirch, France)

Dynamin 2 mutations linked to human diseases impair different cellular functions and highlight its role in development and adult tissues (20')

Ana Zarubica (Marseille, France)

GRASP55 regulates JAM-C expression in endothelial and tumor cells: implication in tumor dissemination (20')

Coffee break

Session 11: Hijacking by pathogens

Chairs: Florence Niedergang and Chiara Zurzolo

Jost Enninga (Paris, France)

The role of host endomembrane organisation during pathogen invasion and the formation of an immune response (20')

Karine Gousset (Paris, France)

Role of Tunneling nanotubes (TNTs) in PrPSc intercellular spreading (20')

Sherri Smith (Paris, France)

Stress, Entamoeba histolytica, and the endoplasmic reticulum (20')

Free time

Poster Session / Annual meeting of Club iTRAFFIc
(Poster listings can be found here)

Dinner

Trafficking Clubs Party

Session 12: Traffic and cytoskeleton

Chairs: Stéphane Gasman and Natalie Sauvonnet

Cyril Basquin (Paris, France)

Role of the PI 3-kinase in the clathrin-caveolin-independent endocytosis used by the IL-2 receptor (20')

Evelyne Coudrier (Paris, France)

Myosin 1b promotes the formation of post-Golgi carriers by regulating actin assembly and membrane remodelling at the trans-Golgi network (20')

Sylvain Loubéry (Geneva, Switzerland)

Kinesin-3 and Uninflatable, two regulators of asymmetric endosomes during asymmetric cell division in Drosophila (20')

Andrew J. Lindsay (Paris, France)

Class V Myosins - the promiscuous actin motors and their Rab partners (20')

Coffee break

Session 13: Organelle biogenesis

Chairs: Evelyne Coudrier and Remi Sadoul

Charlène Delestre (Mont Saint Aignan, France)

Chromogranin A induces the recruitment of cytoskeleton-associated proteins to establish a regulated secretory pathway (20')

Ilaria Palmisano (Milan, Italy)

OA1, an intracellular G protein-coupled receptor, regulates both melanosome biogenesis and transport in pigment cells (20')

Chiara Paiardi (Milan, Italy)

The involvement of endocytic traffic in sperm acrosomogenesis (20')

Cédric Delevoye (Paris, France)

Endocytic kinesin-rabs interactions control the melanosome biogenesis switch (20')

Closing remarks

Departure